Clinical Study 2

OBJECTIVE:
Comparison of epidermal cell proliferation produced by SESHA SKIN THERAPY A.C.E. Cream containing PET™ nutrient delivery system with Retin-A Cream (0.025%) and Oil of Olay Original Beauty Fluid.

PROTOCOL AND STUDY PARAMETERS:
7-day in-vivo study

RESULTS:
The A.C.E Cream significantly thickened the epidermis. The skin is plump and has characteristics of young, healthy skin.

Retin-A 0.025% produced a slight increase of skin cells, but the epidermis is damaged.

Oil of Olay did not show signs of cell proliferation.

CONCLUSION:
Biopsies stained with hematoxylin and eosin at 400x magnification show that A.C.E. Cream is an effective stimulator of epidermal cells.
Study conducted by Peter T. Pugliese, M.D.

Table #1 – Epidermal Thickness in microns (micrometers):

SUBJECT A.C.E . CREAM RETIN-A 0.025% OIL OF OLAY
A  50.3 33 19.8
B 47.3 34 21
C 59.5 39 18
D 58.3 39 21
E 51.5 36 21
MEAN 53.4 36.2 20.2


Hematoxylin and Eosin Stain of sample skin biopsies at 400x :