Science

PET_diagram

P.E.T.® Technology

P.E.T.® works by solubilizing the active ingredients and temporarily modifying the permeability of the skin. Therefore, when large-molecule active ingredients are combined with P.E.T.®, they are able to pass through the skin cell barrier to reach the deeper layers below the epidermis. P.E.T.® is very versatile and allows us to combine nutrients to work together as a networking system. Thus, P.E.T.® enables the penetration of the actives and provide you with the full benefits of each individual ingredient.

P.E.T.® is also extremely versatile and compatible with formulations that containing peptides, vitamins, antioxidants and amino acids enabling deep controlled penetration through the top layer stratum corneum in a single formulation.

Before & After

Client Study - Clinical Line
At the beginning of the study, my main concerns about my skin were dryness, continued aging, and wrinkling. After using SESHA's Clinical line for 3 months, I was more than pleased with the remarkably visible results. Not only was my skin more hydrated, but the fine lines and wrinkles around my eyes and mouth were disappearing! I am delighted with the results and highly recommend SESHA Skin Therapy as a skincare line that really works! - JB
These photos are authentic original images, and have not been retouched or modified in any way. Results may vary based on the user's age, the frequency of application, as well as particular skin or environmental conditions.

Clinical Studies

01

OBJECTIVE:

Evaluation of the efficacy of SESHA SKIN THERAPY A.C.E. Cream with PET™ delivery system.

PROTOCOL AND STUDY PARAMETERS:

Double-blind, 180-day skin test on 15 subjects 50 years and older. For the first 60 days, subjects used either an active or placebo cream. From days 60 to 180 of the study, all subjects were given A.C.E. Cream. Each subject was instructed to use the given product every night on top of their right hand.

Changes in the skin’s epidermal density, thickness, color and wrinkles depth were monitored using ballistometry, ultrasound B-mode scans, photographs, clinical assessments, silastic castings, and skin sample biopsies.

RESULTS:

  • Histological examinations of skin biopsies taken on days 1, 60, 120, and 180 clearly showed that the epidermis becomes thicker with the use of A.C.E. Cream.
  • Epidermal thickness (Masson Stain Slides) increased by 19% or greater over the course of the study with some subject samples showing a 50% increase in epidermal thickness.
  • The stratum corneum (Masson Stain Slides) also shows increased signs of a basket weave pattern that is typical in younger skin.
  • Most subjects showed increased cellular growth of the dermis. This finding is consistent with restructuring process of the dermis.

CONCLUSION:

Ultrasound evaluation of A.C.E treated skin also showed a gradual increase in skin thickness and density for up to three or four months before reaching a steady state. This further supports the proposed proliferation of the epidermis followed by dermal restructuring effect caused by repeated application of A.C.E. Cream.

Study conducted by Peter T. Pugliese, M.D.

02

OBJECTIVE:

Comparison of epidermal cell proliferation produced by SESHA SKIN THERAPY A.C.E. Cream containing PET™  delivery system with Retin-A Cream (0.025%) and Oil of Olay Original Beauty Fluid.

PROTOCOL AND STUDY PARAMETERS:

7-day in-vivo study

RESULTS:

  • The A.C.E Cream significantly thickened the epidermis. The skin is plump and has characteristics of young, healthy skin.
  • Retin-A 0.025% produced a slight increase of skin cells, but the epidermis is damaged.
  • Oil of Olay did not show signs of cell proliferation.

CONCLUSION:

Biopsies stained with hematoxylin and eosin at 400x magnification show that A.C.E. Cream is an effective stimulator of epidermal cells.
Study conducted by Peter T. Pugliese, M.D.

Table #1 – Epidermal Thickness in microns (micrometers):

SUBJECT A.C.E. CREAM RETIN-A 0.025% OIL OF OLAY
A
B
C
D
E
50.3
47.3
59.5
58.3
51.5
33
34
39
39
36
19.8
21
18
21
21
MEAN 53.4 36.2 20.2

Hematoxylin and Eosin Stain of sample skin biopsies at 400x :

03

OBJECTIVE:

Research the effectiveness of Super C Serum and A.C.E. PLUS Cream in reducing fine lines and improving skin texture

VARIABLE:

Super C Serum and A.C.E. PLUS Cream

SUBJECTS:

Six females, between the ages of 37 and 58. All were free of skin diseases as determined by medical history. All showed signs of fine lines and wrinkles as well as redness and/or hyper-pigmentation in the form of freckles or age spots.

PROCEDURE:

This was a 12 week study during which all subjects were instructed to apply Super C Serum over the “crows feet” area around the eyes first, followed by applying A.C.E. PLUS Cream over the entire face. Subjects repeated this in the morning and at nighttime and were evaluated on day 1, week 6 and week 12.

The following parameters were used to measure the performance of the creams: photographs, clinical assessments, silastic castings, and biopsies for histological examinations.

CONCLUSION:

  • Biopsies among those who used Super C Serum and A.C.E. PLUS Cream indicate proliferation in the epidermis and an increase of collagen in the dermis.
  • Castings of lines and wrinkles taken periodically throughout the study show a reduction in the depth and length of wrinkles.
  • All subjects had a marked reduction in redness and an overall improvement in skin tone and elasticity.


Study conducted by Peter P. Pugliese, MD